Abstract: Metarhizium anisopliae infects mosquitoes through the cuticle and proliferates in the hemolymph. To allow M. anisopliae to combat malaria in mosquitoes with advanced malaria infections, we produced recombinant strains expressing molecules that target sporozoites as they travel through the hemolymph to the salivary glands.
Eleven days after a Plasmodium-infected blood meal, mosquitoes were treated with M. anisopliae expressing salivary gland and midgut peptide 1 (SM1), which blocks attachment of sporozoites to salivary glands; a single-chain antibody that agglutinates sporozoites; or scorpine, which is an antimicrobial toxin. These reduced sporozoite counts by 71%, 85%, and 90%, respectively. M. anisopliae expressing scorpine and an [SM1]8:scorpine fusion protein reduced sporozoite counts by 98%, suggesting that Metarhizium-mediated inhibition of Plasmodium development could be a powerful weapon for combating malaria.
Authors:
1. Weiguo Fang, Department of Entomology, University of Maryland
2. Joel Vega-Rodríguez, Malaria Research Institute, Department of Molecular Microbiology and Immunology, Johns Hopkins School of Public Health
3. Anil K. Ghosh, Malaria Research Institute, Department of Molecular Microbiology and Immunology, Johns Hopkins School of Public Health
4. Marcelo Jacobs-Lorena, Malaria Research Institute, Department of Molecular Microbiology and Immunology, Johns Hopkins School of Public Health
5. Angray Kang, Antibody Technology Group, Department of Molecular and Applied Biosciences, School of Life Sciences, University of Westminster
6. Raymond J. St. Leger, Department of Entomology, University of Maryland
Source
Science 25 February 2011:
Vol. 331 no. 6020 pp. 1074-1077
DOI: 10.1126/science.1199115
More information
Full Text: Development of Transgenic Fungi That Kill Human Malaria Parasites in Mosquitoes (PDF)